Figure 6.

Effect of poCLEC12A engagement on the production of cytokines. FACS-sorted pDCs were cultured with anti-poCLEC12A mAb FA2B10, an isotype-matched control mAb or in medium alone, in the presence of either CpG/ODN2216 (5 μg/ml) (A) or poly I:C (10 μg/ml) (B) for 18 h, after which supernatants were collected and analyzed by ELISA for cytokine production. Results shown (mean + SD) are from a representative experiment, run in triplicates (number of performed experiments: 3 for CpG, 2 for poly I:C, performed with cells from different donors).