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. 2020 May 13;11:863. doi: 10.3389/fimmu.2020.00863

Table 1.

Oligonucleotide primers used in this work.

Gen Primer Sequence TAN °C bp
CLEC12A-Nt-GFP C12NtGFPF ATG TCT GAA GAA GTC ACT TAT GCA GAT C 58 792/639
C12NtGFPR CTA CAT TCT TCC AGC TGG TTC TTC AC
CLEC12A-Fc ECD C12F ATT AGC TAG C GA AAA GTT GAA TAA ACT ACA AAA TTT CAA TGA AGA ACT TC 5 c 58°/30 c 62° 614
ECD C12R ATA T TC TAG A CA TTC TTC CAG CAG GTT CTT CAC TTG TTA GTA TAT CC
SP F ATT AGC TAG CCT TGA GCT GAA AAT GAT GTG CAG TAG C 5 c 56°/30 c 63° 122
SP R ATT AGC TAG CAT TGC TTG CTG CTT CTG ACT TGC