FIGURE 1.

BRG1 is essential for endothelial-mesenchymal transition in vitro. (A) HUVECs were transfected with siRNA targeting BRG1 (siBRG1) or scrambled siRNA (SCR). BRG1 expression was examined by qPCR and Western. (B,C) HUVECs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with TGF-β. Gene expression levels were examined by qPCR and Western. (D,E) HUVECs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with hypoxia. Gene expression levels were examined by qPCR and Western. Error bars represent SEM (*p < 0.05, two-way ANOVA with post-hoc Scheffe test). All experiments were repeated three times and data represent averages of three independent experiments.