FIG 4.

Photoreactivation of UVB-inactivated conidia by RAD23 interacting with PHR2. (A) Relative transcript (RT) levels of phr1 and phr2 in the 3-day-old SDAY cultures of rad23 mutants versus the WT. (B) Yeast two-hybrid assay for an interaction of RAD23 with PHR1 or PHR2. Note that only the yeast cell type expressing AD-PHR2-BD-RAD23 grew as well as the positive control (AD-LargeT-BD-P53) on quadruple-dropout synthetic defined medium (SD) (left), whereas all four cell types were able to grow on double-dropout SD (right). (C) RT levels of phr2 in the 3-day-old SDAY cultures of three Δrad23::phr2 strains versus the WT. (D) LD₅₀ estimates for conidial UVB resistances of three Δrad23::phr2 strains, WT, and phr2 mutants. (E) Photoreactivated (germinated) percentages of UVB-inactivated conidia. The asterisked bar in each bar group (in panel A) differs significantly from those that are unmarked, and different lowercase letters (in panels D and E) also denote significant differences (Tukey’s HSD, P < 0.05). Error bars indicate standard deviations from three replicates.