FIG 1.

Workflow for preparing the nonlive Csp_P biopesticide. (A) Prepare large plates with 200 ml of LB agar and then inoculate each with 200 μl of Csp_P culture. (B) Allow plates to grow for 2 days at 30°C and then inoculate with 200 ml of LB broth. (C) Grow for 5 days and then decant the liquid phase. Let plates sit for 24 h and then scrape off the bacterial lawn. (D) Collect bacterial lawn in a petri dish. (E) Air dry preparation in fume hood. (F) Once dry, crush the preparation to powder with a mortar and pestle. (G) Incorporate nonlive Csp_P powder into gelatin/fishmeal attractive pellets. (H) Feed pellets containing 100 mg of powder to target mosquito larvae.