FIG 4.

Nonlive Csp_P powder effectively kills mosquito larvae under semi-field conditions. Ae. aegypti Patillas (A) and Ae. mediovittatus (B) larvae were reared under insectary conditions for 3 days and then transferred to a semi-field facility at Gurabo, Puerto Rico. Larvae were treated with a no-bacterium control pellet (black lines) or a pellet containing 200 mg of nonlive Csp_P (blue lines) and then left under ambient environmental conditions. We observed significant mortality induced by nonlive Csp_P for larvae from both species (Cox regression: P < 0.0001), indicating that the larvicide performed effectively under semi-field conditions. Larvae were reared in groups of 30 in 300 ml of tap water. Lines depict the percentages of larvae surviving at each day posttreatment (± SEM).