FIG 5.

Nonlive Csp_P powder effectively kills larvae from different Ae. aegypti G₁ field-derived populations under semi-field conditions. Ae. aegypti eggs were collected from 11 different sites around Puerto Rico using oviposition cups. Egg papers were hatched under insectary conditions, typed for mosquito species, and then transferred to the semi-field cage. Larvae from each population were divided in two, with half fed a control pellet (black lines) and half fed a pellet containing 200 mg of nonlive Csp_P (blue lines), and then left under ambient environmental conditions. A 100% mortality for all Csp_P-treated larvae was achieved in 2 to 6 days. Each panel depicts a different Ae. aegypti population: Bayamon (A), Catano (B), Guayanilla (C), Gurabo (D), Humacao (E), Juncos (F), Loiza (G), Ponce (H), Toa Alta (I), Trujillo Alto (J), and Yauco (K). Larvae were reared in groups of 7 to 15 in 300 ml of tap water. Lines depict the percentages of larvae surviving at each day posttreatment.