FIG 3.
SDS gel of extracts from T. chlorobenzoica 3CB-1 grown aerobically/anaerobically with phthalate/benzoate (10% acrylamide). Lane 1, phthalate/O2; lane 2, phthalate/nitrate; lane 3, benzoate/O2; lane 4, benzoate/nitrate (15 μg protein per lane); M, molecular mass standards. The arrow labels refer to proteins bands that were excised, tryptically digested, and analyzed by UPLC-ESI-Q-TOF mass spectrometry. The results of these analyses are summarized in Table S1 in the supplemental material. The assigned proteins are the following: benzoyl-CoA dihydrodiol lyase (component BoxC) (A), PCD (B), benzoyl-CoA monooxygenase component BoxB (C), benzoyl-CoA monooxygenase component BoxA (D), benzoate CoA ligase/NirS nitrite reductase component (E), and UbiX-like prenyltransferase (F).