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Enrichment of PCD from extracts of T. chlorobenzoica 3CB-1 grown with phthalate/O2a
| Enzyme or enrichment step | Total protein (mg) | Total activity (nmol min–1) | Specific activity (nmol min–1 mg–1) (mean ± SD) | Enrichment (fold) | Recovery (%) |
|---|---|---|---|---|---|
| 100,000 × g supernatant | 356 | 15,240 | 42.8 ± 8 | 1 | 100 |
| DEAE-Sepharose | 85 | 7,760 | 91.2 ± 20 | 2.1 | 51 |
| Gel filtration | 27.5 | 3,980 | 145 ± 32 | 3.4 | 26 |
PCD was purified from 4.25 g (wet mass) of Thauera chlorobenzoica cells.
