FIG 5.

AcFur SI is important for iron transport. (A) The AcFur box of A. caldus MTH-04, predicted by using Virtual Footprint and gene locations. (B) Binding of AcFur to the feoABC operator and the feoP promoter at different concentrations of AcFur. (C) Expression of feoA and feoP genes in wild-type A. caldus strain monitored by qRT-PCR after acid shock. (D and E) Binding of the feoABC operator and the feoP promoter, respectively, to the AcFur, SI, SII, and SI plus SII at different concentrations of protein. (F and G) Expression levels of feoA and feoP in the fur knockout strain and Δfur(fur), Δfur(SI), Δfur(SII), and Δfur(SI&SII) strains, tested separately by qRT-PCR. Error bars show standard deviations.