TABLE 2.
Strains and plasmids used in this study
| Strain, mutant, or plasmid | Descriptiona | Reference or source |
|---|---|---|
| E. coli strain | ||
| DH5α | Strain used for gene cloning | TaKaRa |
| C. hutchinsonii strains | ||
| ATCC 33406 | Wild type | ATCC |
| ΔgldN | chu_0174 deleted | This study |
| Δ2610 | chu_2610 deleted | This study |
| C0174 | Complementation of ΔgldN with pHB0174 | This study |
| Δ2807 | chu_2807 deleted | This study |
| Δ0344 | chu_0344 deleted | 20 |
| Δ0052 | chu_0052 deleted | Unpublished data |
| Δ0007 | chu_0007 deleted | This study |
| Δ3384 | chu_3384 deleted | This study |
| Δ3732 | chu_3732 deleted | 41 |
| Plasmids | ||
| pCFX | Gene deletion template plasmid carrying cfxA flanked by two MCS; Apr (Cfxr); derived from pCFXSK with removal of the FRT sites | This study |
| pTSKQ | Gene deletion template plasmid carrying ermF flanked by two MCS; Apr (Emr); derived from pTSK with removal of the FRT sites | This study |
| pTKS | Gene deletion template plasmid carrying ermF flanked by two MCS; Apr (Emr) | 20 |
| pSJHS | Gene deletion template plasmid carrying ermF flanked by two MCS; Apr (Emr) | 20 |
| pSKSO8TG | Used for complementation of the mutants in C. hutchinsonii with oriC; Apr (Emr) | 20 |
| pHB0174 | Plasmid constructed from pSKSO8TG used for complementation of ΔgldN; Apr (Emr) | This study |
| pHB2807 | Plasmid constructed from pSKSO8TG used for complementation of Δ2807; Apr (Emr) | This study |
a
MCS, multiple cloning site(s); FRT, FLP recombination target; Apr, ampicillin resistance; Cfxr, cefoxitin resistance; Emr, erythromycin resistance. Phenotypes in parentheses are expressed in C. hutchinsonii, and phenotypes not in parentheses are expressed in E. coli.