Figure 2.

Generation and characterization of M-opsin gene deficient (Opn1mw^−/−) mice. A: A schematic illustration on the location of the single-guide RNA targeting sequence in the mouse Opn1mw gene. B: Western blot analysis of M-opsin and S-opsin from 1-month–old wild-type (WT) and Opn1mw^−/− retinas. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as a loading control. C: DNA sequencing traces of WT and Opn1mw^−/− mice. There was a 1-bp insertion (arrow) at the targeting site, which caused a frameshift and truncation (Supplemental Figure S1). D: Immunolabeling of M-opsin and S-opsin in the retinal sections of 1-month–old WT and Opn1mw^−/− mice. M-opsin and S-opsin were imaged from the dorsal and ventral retina, respectively. Nuclei were stained with DAPI (blue). Scale bars = 10 μm. CIS, cone inner segment; COS, cone outer segment; ONL, outer nuclear layer; OPL, outer plexiform layer.