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. 2020 May;190(5):1018–1029. doi: 10.1016/j.ajpath.2020.01.013

Figure 5.

Figure 5

Determination of hepatic fibrosis and hepatic stellate cell (HSC) activation. HSC activation was determined by immunofluorescence for desmin [co-stained with cytokeratin (CK)-19 to mark bile ducts] and hepatic fibrosis by staining for Fast Green/Sirius Red (and semiquantification) and immunohistochemistry for vimentin. A: The intensity of desmin increases in Mdr2−/− (ATP binding cassette subfamily B member 4 null) mice treated with mismatch compared with wild-type (WT) mismatch; however, Mdr2−/− mice treated with histamine-2 receptor (H2HR) vivo-morpholino have a reduction in desmin staining. B: Similar trends are seen for Fast Green/Sirius Red staining that displays significantly increased amounts of collagen deposition around the portal area in Mdr2−/− mice treated with mismatch compared with WT mismatch. Mdr2−/− mice that were treated with H2HR vivo-morpholino have less collagen deposition. Fast Green/Sirius Red is also shown semiquantified. C: Vimentin staining reveals a number of vimentin-positive cells surrounding the portal area in WT groups that is increased in Mdr2−/− mice treated with mismatch; however, there are fewer vimentin-positive cells in Mdr2−/− mice treated with H2HR vivo-morpholino. ∗P < 0.05 versus WT mismatch; †P < 0.05 versus Mdr2−/− + H2HR mismatch. Original magnification: ×40 (A–C); ×80 (C, insets).