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. 2020 May;190(5):1018–1029. doi: 10.1016/j.ajpath.2020.01.013

Figure 7.

Figure 7

Evaluation of angiogenesis. Vascular endothelial growth factor (VEGF)-A and -C expression was determined by immunofluorescence in all groups of mice, and VEGF-A levels were measured in large cholangiocyte supernatant and serum. Wild-type (WT) mice treated with histamine-2 receptor (H2HR) vivo-morpholino have less biliary expression of VEGF-A (VEGF-C expression was similar in WT groups), and VEGF-A (A) and VEGF-C (B) expression increases in large bile ducts [stained with cytokeratin (CK)-19] from multidrug-resistance transporter 2/ABC transporter B family member 2 knockout (Mdr2−/−) mice treated with mismatch that is reduced in Mdr2−/− mice treated with H2HR vivo-morpholino. Cholangiocyte supernatant (C) and serum secretion of VEGF-A (D) increase in Mdr2−/− mice treated with mismatch that is significantly reduced in Mdr2−/− mice treated with H2HR vivo-morpholino. No changes are seen in the WT groups. Data are expressed as means ± SEM. n = 9experiments for enzyme immunoassay. ∗P < 0.05 versus WT mismatch; †P < 0.05 versus Mdr2−/− mice + H2HR vivo-morpholino. Original magnification, ×40 (A and B).