Figure 8.

Determination of intracellular cAMP/ERK signaling. cAMP and ERK expression was evaluated by immunofluorescence [co-stained with cytokeratin (CK)-19 to mark bile ducts). Both cAMP (A) and phospho-ERK (B) expression and co-localization increase in large bile ducts (arrows) in Mdr2^−/− (ATP binding cassette subfamily B member 4 null) mice treated with mismatch, which is reduced in multidrug-resistance transporter 2/ABC transporter B family member 2 knockout (Mdr2^−/−) mice treated with H2HR vivo-morpholino. There are no changes between wild-type (WT) groups. Original magnification: ×40 (A, top row); ×80 (A, bottom row, and B); ×100 (A and B, enlargements).