Figure 6.

SRSP promotes the inclusion of exon 3 of Sp4. A) L‐Sp4 and S‐Sp4 splicing variants were determined by RT‐PCR in LOC90024 KO HeLa cells. B) HCT‐116 cells were transfected with two anti‐LOC90024 siRNAs, and levels of L‐Sp4 and S‐Sp4 splicing variants were determined. C) The indicated LOC90024 constructs were transfected into HCT‐116 cells, and levels of L‐Sp4 and S‐Sp4 splicing variants were determined. D) The indicated LOC90024 constructs were transfected into LOC90024‐KO HeLa cells, and levels of L‐Sp4 and S‐Sp4 splicing variants were determined. E) HCT‐116 cells were transfected with SRSP vector together with anti‐SRSF3 siRNA, and levels of L‐Sp4 and S‐Sp4 splicing variants were determined. F) Levels of L‐Sp4 and S‐Sp4 splicing variants were determined between T and their matched NT. G) The relationships of LOC90024 lncRNA levels with L‐Sp4 (upper panel) and S‐Sp4 (low panel) mRNA levels were analyzed in clinical tissue samples. Data are represented as mean ± SD. ***p < 0.001.