Figure 4.

Single-Cell Gene Expression Profiles Map the Spatial Organization of the Embryo

(A) Projection on Axis16₁ and Axis16₂ of single-cell transcriptomes of all 16 cells of a 16-cell embryo. Inset: 16-cell embryo side view.

(B) Mapping of cell types in a 16-cell embryo.

(C) Projection on Axis16₁ and Axis16₂ of single-cell transcriptomes of 29 cells of a 32-cell embryo. Inset: 32-cell embryo side view.

(D) Mapping of cell types in a 32-cell embryo.

(E and F) (E) or of A lineage cell types on the classifier of the daughters of A6.1 and 3 and A6.2 and 4 and the direction of the remainder of the variance in the A lineage. (F). Inset: 64-cell embryo vegetal pole. B7.6 belongs to the germ cell lineage. Dashed lines link sister cell types.

(G) Mapping of cell types in a 64-cell embryo.

See also Figures S4 and S5 and Table S2.