Figure 5.

Integration of Imaging and scRNA-Seq Data Identify Patterned Protocadherin Expression

(A) Relative apical surface of cells (p = 0.002 at 16-cell stage and p = 7.10⁻⁶ at 32-cell stage, Kruskal-Wallis test).

(B) Expression territories of four protocadherins.

(C) Comparison between the estimated protocadherin-11 X-linked contact strength and the relative apical surface in animal cells (r = −0.94 and p = 0.0006 at 16-cell stage, r = −0.67 and p = 0.004 at 32-cell stage, Pearson’s correlation coefficient).

(D) Average relative apical surface of animal and vegetal cells of control embryos and embryos treated with Ca²⁺-free seawater at 32-cell stage (p = 1.5 10⁻⁶ for the animal and vegetal comparison in control embryos, n = 4; p = 0.18 for the animal and vegetal comparison in Ca²⁺-free-treated embryos, n = 3; p = 0.01 for the animal cells control/Ca²⁺-free comparison; p = 0.17 for the vegetal cells control/Ca²⁺-free comparison; n.s., non-significant; two-tailed t test; data represented as mean ± SD).

See also Figure S6.