Figure S6.

Morphological Changes and Patterned Expression of Cell Adhesion Molecules, Related to Figure 5

(A) Comparison between cell volume and surface as measured by SPIM imaging from 2-cell to 64-cell stages (r = 0.97 and p = 2.10⁻⁷⁶, Pearson’s correlation coefficient).

(B) Temporal evolution of the relative apical surface from 8-cell to 16-cell stage as measured by SPIM imaging.

(C) Temporal evolution of the apical contact perimeter from 8-cell to 16-cell stage as measured by SPIM imaging.

(D) Expression levels of four protocadherins in the different cell types.

(E) Relative apical surface of vegetal cells at the 32- and 64-cell stages.

(F) Expression territories of six cadherins and protocadherins at the 64-cell stage as measured by scRNA-Seq. Territories were displayed on rendered segmented membrane signal from SPIM imaging.

(G) Comparison between the estimated cadherin-7 contact strength and the relative apical surface in animal cells at the 64-cell stage (r = −0.62 and p = 0.0001, Pearson’s correlation coefficient).

(H) Relative apical surface of animal and vegetal cells at 32-cell stage of control embryos and embryos treated with Ca²⁺-free seawater.