Figure 1.

IL-33 Upregulates Hemin-Induced Spic Expression and Promotes the Development of a Red Pulp Macrophage (RPM) Phenotype In Vitro and In Vivo

(A) Gene expression in mouse bone-marrow-derived macrophages stimulated in vitro for 4 days with hemin (40 μM), IL-33 (10 ng/mL), or a combination of hemin + IL-33, compared to no treatment (NT). Data represent mean ± SEM and are representative of five independent experiments. ^∗∗p < 0.01, ^∗∗∗∗p < 0.001.

(B–E) Representative examples (B) and quantification (among CD45⁺ CD11c^low Ly6G^low NK1.1^low SSC-A^low cells) of flow cytometry staining for splenic monocytes (CD11b⁺ F4/80⁻) (C), pre-RPMs (CD11b⁺ F4/80^lo) (D), and RPMs (CD11b^lo/− F4/80^hi) (E) in mice injected intraperitoneally once a day for 3 days with either phosphate-buffered saline (PBS), IL-33 (1 μg), hemin (500 μg), or IL-33 + hemin. ^∗∗∗∗p < 0.001. Data representative of at least five independent experiments.

Please also see Figure S1.