Figure 2.

IL1RL1 Signaling Controls the Development of Splenic Red Pulp Macrophages (RPMs)

(A) Quantification (percentage among CD11c^low Ly6G^low NK1.1^low SSC-A^low cells) of splenic pre-RPMs (CD11b^hi F4/80^lo) and RPMs (CD11b^lo/− F4/80^hi) in neonates (Day 1), young (6 weeks), and old (42 weeks) WT and Il1rl1^−/− mice. Each dot represents a separate mouse. ^∗∗∗∗p < 0.001.

(B) Staining of RPMs (F4/80 shown in green) in spleen sections of 42-week WT and Il1rl1^−/− mice.

(C) Flow cytometry staining and quantification of splenic RPMs (CD11b^lo/Spic-EGFP^hi) and pre-RPMs (CD11b⁺Spic-EGFP^lo/int) (percentages among CD11c^low Ly6G^low NK1.1^low SSC-A^low cells) after 6 weeks of treatment of Spic^igfp/igfp reporter mice with control murine IgG1 (mIgG1) or soluble IL1RL1-murine Fc fusion protein (soluble IL1RL1). Each dot in the quantification panels represents a separate mouse. Data represent mean ± SEM. ^∗∗∗∗p < 0.001.

Please also see Figures S2 and S3.