Figure 6.

GATA2 Is Implicated in the Development of Splenic Red Pulp Macrophages (RPMs) and Differentially Expressed in WT and Ilrl1^−/− Pre-RPMs

(A)) Heatmap showing relative expression of genes differentially expressed between WT monocytes and WT pre-RPMs (693 genes, log2 fold change > 1, adjusted p value < 0.01) in splenic monocytes (CD11b^hi F4/80⁻) and pre-RPMs (CD11b^hi F4/80^lo) from WT and Il1rl1^−/− mice; n = 4 separate mice per group. (B) Heatmap showing relative expression of genes differentially expressed between WT pre-RPMs and WT RPMs (3079 genes, log2 fold change > 1, adjusted p value < 0.01) in pre-RPMs (CD11b^hi F4/80^lo) and RPMs (CD11b^lo/− F4/80^hi) from WT and Il1rl1^−/− mice; n = 4 separate mice per group

(C) Top upstream transcriptional regulators for transcriptomic changes from monocytes to pre-RPMs, or pre-RPMs to RPMs, in WT and Il1rl1^−/− mice, identified by Ingenuity pathway analysis. Note: the top regulators in each group were significant in both groups.

(D) Top differentially expressed genes in pre-RPMs (CD11b^hi F4/80^lo) from WT and Il1rl1^−/− mice; n = 4 separate mice per group.

Please see also Table S2 and Figure S7.