Figure 7.

A GATA Switch May Underlie Changes in Gene Expression in the Development of Splenic Red Pulp Macrophages (RPMs)

(A) Identification of GATA motifs in accessible chromatin of splenic monocytes (CD11b^hi F4/80⁻), pre-RPMs (CD11b^hi F4/80^lo), and RPMs (CD11b^lo/− F4/80^hi), within 100 kb of the start sites of genes differentially expressed between WT monocytes and WT pre-RPMs, or WT pre-RPMs and WT RPMs (both log2 fold change > 1, adjusted p value < 0.05). Motif enrichment was performed on accessible chromatin from each splenocyte population from WT and Il1rl1^−/− mice and also from accessible chromatin from each splenocyte population that was unique to WT or unique to Il1rl1^−/− mice; n = 4 separate mice per group.

(B) Relative Gata gene expression (normalized counts, cpm) in splenic monocytes (CD11b^hi F4/80⁻), pre-RPMs (CD11b^hi F4/80^lo), and RPMs (CD11b^lo/− F4/80^hi) of WT (green) and Il1rl1^−/− mice (red); n = 4 separate mice per group. Data are represented as mean ± SEM. ^∗∗∗∗ adjusted p (WT v −/−) < 0.001.

(C) Heatmaps showing log2 fold changes of genes differentially expressed in WT and Il1rl1^−/− pre-RPMs (adjusted p value < 0.05) between splenic monocytes (CD11b^hi F4/80⁻) and pre-RPMs (CD11b^hi F4/80^lo), or pre-RPMs (CD11b^hi F4/80^lo) and RPMs (CD11b^lo/− F4/80^hi), from WT and Il1rl1^−/− mice; n = 4 per group.

Please see also Figure S7.