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. 2020 May 14;181(4):818–831.e19. doi: 10.1016/j.cell.2020.04.009

Figure S7.

Figure S7

Condensation of Ded1p in Response to Elevated Temperature and Lowered pH Is Conserved in Three Fungi, Related to Figure 7

(A) Coomassie stained SDS-PAGE of purified Ded1-GFP from S. kudriavzevii (S.k.), S. cerevisiae (S.c.) and T. terrestris (T.t.).

(B) Light scattering of purified Ded1-GFP homologs in PIPES/KOH pH 6.8, 100 mM KCl buffer. Temperature ramp 1°C/min.

(C) Representative microscopy images of Ded1-GFP homologs in PIPES/KOH pH 6.8 or PIPES/KOH pH 5.7, 100 mM KCl buffer. Scale bar, 3 μm.