Skip to main content
. 2020 May 19;52(5):794–807.e7. doi: 10.1016/j.immuni.2020.03.010

Figure 3.

Figure 3

Homing Paths of T Cells Are Guided by Blood Vessels

(A–D) Snapshots from intravital imaging of live hCD2-DsRed spleens by TPLSM.

(A) Left, 3D view of a 70-μm z-projection. Right panels, XY (top) and ZY (bottom) are views of a magnified part of the same area.

(B) 105-μm z-projection of endogenous T cell (red) and collagen (second harmonic, white). Data in (A) and (B) are representative of at least 20 mice and 40 videos recorded.

(C) 3D view of 27-μm z-projection of a spleen a few minutes after intravenous injection of Dextran-FITC 2,000 kDa (green). Data are representative of at least three mice and six videos acquired. See also Video S5.

(D) 18-μm z-projection of GFP+ red blood cells (RBC, green) injected into a hCD2-DsRed host. Data are representative of at least six mice and 14 videos acquired. See also Video S6.

(E) Fixed spleens from hCD2-DsRed mice were sliced with a vibratome (150 μm thick) and stained for CD31. Micrographs show high magnifications of areas highlighted in dotted box. Sections were imaged by TPLSM. Data represent sections from at least three mice analyzed.