Figure 6.

CCR7 Is Dispensable for T Cell Access to the PT-Tracks, but Required for Directional Migration and Entry into the T Zone

(A) 84-μm z-projection (left) and time-projection (right) view showing migration of wild-type (WT, white) and Ccr7^−/− (green) T cells (green) 24 h after transfer into a hCD2-DsRed recipient. Grey line highlights PT-tracks connected to a T zone. Yellow dotted line indicates the “upper perimeter of the T zone” area, where Ccr7^−/− accumulate. See also Video S8.

(B) Trajectories of transferred WT and Ccr7^−/− T cells moving toward the T zone (marked in cyan) and away from it (purple). Right, summary of frequencies of cells migrating toward or away from the T zones. Each circle represents the average frequency measured in one mouse. Error bars represent SD.

(C) Mean velocities and straightness of cell trajectories of transferred T cells migrating along PT-tracks. The figure shows results from four independent experiments in which WT and Ccr7^−/− T cells were co-transferred. These data are consistent with those observed in four additional experiments where Ccr7^−/− T cells were transferred alone (data not shown). Error bars represent SD.

(D) 105-μm z-projection view (left) and T cell tracks (right) showing migration of control PBS- (white) and pertussis toxin (PTX)-treated T cells (green) transferred into hCD2-DsRed recipient.

(E) Frequencies of transferred cells associated with PT-tracks (right).

Data are representative of five videos and three mice. Error bars represent SD. ^∗∗∗∗p < 0.0001; ^∗∗∗p < 0.001; ^∗p < 0.05.