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. 2020 May 20;181(7):1489–1501.e15. doi: 10.1016/j.cell.2020.05.015

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Figure S2 — Phenotyping Flow Cytometry, Related to Figure 1

Representative gating of CD3⁺ T cells, CD19⁺ B cells, CD3^-CD19^- cells, CD4⁺ T cells, CD8⁺ T cells and CD14⁺ monocytes from donor PBMCs is shown. Briefly, mononuclear cells were gated out of all events followed by subsequent singlet gating. Live cells are gated as Zombie UV^-. Cells were then gated as CD19-PE-Cy5⁺, CD3-buv395⁺ or CD19^-CD3^- cells. T cells were further subdivided into either CD8-buv805⁺ or CD4-PerCPefluor710⁺ populations. CD3^-CD19^- cells were defined as CD56-PE-Dazzle^bright NK cells, CD56^dimCD-16buv737⁺ NK cells or CD56^- monocytes. Monocytes were further classified on differential expression of CD14-bv510 and CD16.