Figure S4.

SARS-CoV-2-Specific CD8⁺ T Cell Responses of Recovered COVID-19 Patients, Related to Figure 3

(A) Flow cytometry gating strategy.

(B) SARS-CoV-2−specific CD8⁺ T cells as determined by AIM⁺ (CD69⁺CD137⁺) CD8⁺ T cells. Response of PBMCs from COVID-19 cases between the negative control (DMSO) and antigen specific stimulation.

(C) CMV-specific CD8⁺ T cells as percentage of AIM⁺ (CD69⁺CD137⁺) CD8⁺ T cells after stimulation of PBMCs with CMV peptide pool. Data were background subtracted against DMSO negative control and are shown with geometric mean and geometric standard deviation. Samples were from unexposed donors (“Unexposed,” n = 11) and recovered COVID-19 patients (“COVID-19,” n = 10).

(D-E) Stimulation index quantitation of AIM⁺ (CD69⁺CD137⁺) CD8⁺ T cells; the same samples as in Figure 2 and Figure S4C were analyzed.

Statistical comparisons across cohorts were performed with the Mann-Whitney test, while paired sample comparisons were performed with the Wilcoxon test. ^∗∗p < 0.01; ^∗∗∗p < 0.001. ns not significant.