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. 2020 May 20;181(7):1489–1501.e15. doi: 10.1016/j.cell.2020.05.015
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

M5E2 (V500) [anti-CD14] Becton Dickinson 561391 (RRID:AB_10611856)
HIB19 (V500) [anti-CD19] Becton Dickinson 561121 (RRID:AB_10562391)
FN50 (BV605) [anti-CD4] Becton Dickinson 562989 (RRID:AB_2737935)
RPA-T8 (BV650) [anti-CD8] BioLegend 301042 (RRID:AB_2563505)
FN50 (PE-CF594) [anti-CD69] Becton Dickinson 562617 (RRID:AB_2737680)
Ber-ACT35 (PE-Cy7) [anti-OX40] Biolegend 350012 (RRID:AB_10901161)a
4B4-1 (APC) [anti-CD137] BioLegend 309810 (RRID:AB_830672)
OKT3 (AF700) [anti-CD3] Biolegend 317340 (RRID:AB_2563408)
G043H7 (BV421) [anti-CD45RA] BioLegend 353207 (RRID:AB_10915137)
4S.B3 (FITC) [anti-IFNγ] Thermo Fisher Scientific 11-7319-82 (RRID: AB_465415)
GB11 (PE) [anti-Granzyme B] Thermo Fisher Scientific 12-8899-41 (RRID: AB_1659718)
Mab11 (PeCy7) [anti-TNFα] ebioscience 25-7349-82 (RRID:AB_469686)
JES3-19F1 (APC) [anti-IL-10] BioLegend 506807 (RRID:AB_315457)
3D12 (APC ef780) [anti-CCR7] eBioscience 47-1979-42 (RRID:AB_1518794)
B56 (FITC) [anti-KI67] Becton Dickinson 556026 (RRID:AB_396302)
SK3 (percp efluor710) [anti-CD4] Invitrogen 46-0047-42 (RRID:AB_1834401)
GB11 (af647) [anti-GzmB] Biolegend 515406 (RRID:AB_2566333)
MHM-88 (af700) [anti-IgM] Biolegend 314538 (RRID:AB_2566615)
O323 (APC cy7) [anti-CD27] Biolegend 302816 (RRID:AB_571977)
IA6-2 (PE) [anti-IgD] Becton Dickinson 555779 (RRID:AB_396114)
HCD56 (PE Dazzle) [anti-CD56] Biolegend 318348 (RRID:AB_2563564)
HIB19 (Cy5) [anti-CD19PE] Biolegend 302210 (RRID:AB_314240)
HIT2 (PECy7) [anti-CD38] Invitrogen 25-0389-42 (RRID:AB_1724057)
J252D4 (bv421) [anti-CXCR5] Biolegend 356920 (RRID:AB_2562303)
63D3 (bv510) [anti-CD14] Biolegend 367123 (RRID:AB_2716228)
HI100 (bv570) [anti-CD45RA] Biolegend 304132 (RRID:AB_2563813)
G025H7 (bv605) [anti-CXCR3] Biolegend 353728 (RRID:AB_2563157)
2H7 (bv650) [anti-CD20] Biolegend 302336 (RRID:AB_2563806)
G043H7 (bv711) [anti-CCR7] Biolegend 353228 (RRID:AB_2563865)
EH12.2H7 (bv786) [anti-PD-1] Biolegend 329930 (RRID:AB_2563443)
UCHT1 (buv395) [anti-CD3] Becton Dickinson 563546 (RRID:AB_2744387)
Live/dead (UV) [Zombie] Biolegend 423108
11A9 (buv496) [anti-CCR6] Becton Dickinson 612948 (RRID:AB_2833076)
3G8 (buv737) [anti-CD16] Becton Dickinson 612786 (RRID:AB_2833077)
SK1 (buv805) [anti-CD8] Becton Dickinson 612889 (RRID:AB_2833078)
LEGENDplex 13-plex kit Biolegend 740809

Biological Samples

Healthy donor blood samples Carter BloodCare http://www.carterbloodcare.org/
Healthy donor blood samples LJI Clinical Core https://www.iedb.org/
Convalescent donor blood samples UC San Diego Health http://www.health.ucsd.edu/

Chemicals, Peptides, and Recombinant Proteins

Synthetic peptides Synthetic Biomolecules (aka A&A) http://www.syntheticbiomolecules.com/
SARS-CoV-2 Receptor Binding Domain (RBD) protein Stadlbauer et al., 2020 N/A

Critical Commercial Assays

CoronaCheck COVID-19 Rapid Antibody Test Kit 20/20 BioResponse https://coronachecktest.com/

Deposited Data

Wuhan-Hu-1 RNA isolate NCBI nuccore database GenBank: MN_908947
ORF10 protein NCBI protein database NCBI: YP_009725255.1
Nucleocapsid phosphoprotein NCBI protein database NCBI: YP_009724397.2
ORF8 protein NCBI protein database NCBI: YP_009724396.1
ORF7a protein NCBI protein database NCBI: YP_009724395.1
ORF6 protein NCBI protein database NCBI: YP_009724394.1
membrane glycoprotein NCBI protein database NCBI: YP_009724393.1
envelope protein NCBI protein database NCBI: YP_009724392.1
ORF3a protein NCBI protein database NCBI: YP_009724391.1
surface glycoprotein NCBI protein database NCBI: YP_009724390.1
orf1ab polyprotein NCBI protein database NCBI: YP_009724389.1

Software and Algorithms

IEDB Vita et al., 2019 https://www.iedb.org
IEDB-AR (analysis resource) Dhanda et al., 2019 http://tools.iedb.org
NetMHCpan EL 4.0 Jurtz et al., 2017 http://tools.iedb.org/mhci/
IEDB Vita et al., 2019 https://www.iedb.org
Tepitool Paul et al., 2016, Paul et al., 2015 http://tools.iedb.org/tepitool/
FlowJo 10 FlowJo https://www.flowjo.com/
GraphPad Prism 8.4 GraphPad https://www.graphpad.com/
LEGENDplex v8.0 Biolegend https://www.biolegend.com/