Table 1.
Glycosome Conserved Enzyme Collection (GCEC). Proteins from Trypanosoma cruzi, Trypanosoma brucei, and Leishmania donovani global studies of glycosome composition, evidence of localization of endogenous tagged proteins from the TrypTag project, and historical input of glycosome proteins in PeroxisomeDB culled from individual studies prior to 2010. Proteins are grouped by major KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway inclusion. Presence of a targeting signal within the protein is indicated in the PTS1/PTS2 (Peroxisome Targeting Sequence 1/2) column (glycosome PTS1 or conserved peroxisome targeting signal 2, PTS2). It was not possible to assign a best KEGG reference orthologue for all proteins.
| KEGG Reference Ortholog | Major KEGG Pathway | Name of Protein | PTS1/PTS2 |
|---|---|---|---|
| K18561 | Glycolysis | NADH-dependent fumarate reductase | PTS1 |
| Glycolysis | UDP-glc 4’-epimerase | PTS1 | |
| K00850 | Glycolysis | ATP-dependent 6-phosphofructokinase, glycosomal | PTS1 |
| K00844 | Glycolysis/gluconeogenesis | Hexokinase 1 | PTS2 |
| K01810 | Glycolysis/gluconeogenesis | Glucose-6-phosphate isomerase | PTS1 |
| K00134 | Glycolysis/gluconeogenesis | Glyceraldehyde 3-phosphate dehydrogenase | PTS1 |
| K01006 | Glycolysis/gluconeogenesis | Pyruvate phosphate dikinase | PTS1 |
| K00927 | Glycolysis/gluconeogenesis | Phosphoglycerate kinase | PTS2 |
| K01792 | Glycolysis/gluconeogenesis | Aldose 1-epimerase | PTS1 |
| K01623 | Glycolysis/gluconeogenesis | Fructose-bisphosphate aldolase | |
| K01803 | Glycolysis/gluconeogenesis | Triose phosphate isomerase | |
| K03841 | Gluconeogenesis | Fructose-1,6-bisphosphatase | PTS1 |
| K01610 | Gluconeogenesis | Phosphoenolpyruvate carboxykinase [ATP] | PTS1 |
| K00026 | Gluconeogenesis | Glycosomal malate dehydrogenase | PTS1 |
| Gluconeogenesis | UDP-glucose pyrophosphorylase | ||
| K00760 | Purine metabolism | Hypoxanthine-guanine phosphoribosyltransferase 1 | PTS1 |
| K00760 | Purine metabolism | Hypoxanthine-guanine phosphoribosyltransferase 2 | PTS1 |
| K00088 | Purine metabolism | Inosine-5’-monophosphate dehydrogenase | PTS1 |
| K00942 | Purine metabolism | Guanylate kinase | PTS1 |
| K00759 | Purine metabolism | Adenine phosphoribosyltransferase | PTS1 |
| K01490 | Purine metabolism | AMP deaminase | PTS1 |
| K00939 | Purine metabolism | Adenylate kinase | PTS1 |
| K00088 | Purine metabolism | Guanosine monophosphate reductase | PTS1 |
| K00036 | Pentose phosphate pathway | Glucose-6-phosphate 1-dehydrogenase (G6PD) | |
| K00852 | Pentose phosphate pathway | Ribokinase | |
| K01100 | Pentose phosphate pathway | Sedoheptulose-1,7-bisphosphatase | PTS1 |
| K01619 | Pentose phosphate pathway | Deoxyribose-phosphate aldolase | PTS1 |
| K00615 | Pentose phosphate pathway | Transketolase | PTS1 |
| K01057 | Pentose phosphate pathway | 6-phosphogluconolactonase | |
| K00864 | Glycerophospholipid metabolism | Glycerol kinase | PTS1 |
| K00803 | Glycerophospholipid metabolism | Alkyl-dihydroxyacetone phosphate synthase | PTS1 |
| K00649 | Glycerophospholipid metabolism | Dihydroxyacetonephosphate acyltransferase | PTS1 |
| K00006 | Glycerophospholipid metabolism | Glycerol-3-phosphate dehydrogenase (NAD(+)) | PTS1 |
| K00022 | Fatty acid metabolism | Enoyl-CoA hydratase/Enoyl-CoA isomerase/3- hydroxyacyl-CoA dehydrogenase | PTS2 |
| K08766 | Fatty acid metabolism | Carnitine O-palmitoyltransferase | PTS1 |
| K11262 | Fatty acid metabolism | Acetyl-CoA carboxylase | |
| K11207 | Redox maintenance | Trypanothione/tryparedoxin dependent peroxidase 2 | |
| K01833 | Redox maintenance | Trypanothione synthetase | |
| K00103 | Redox maintenance | L-galactonolactone oxidase | PTS1 |
| K00869 | Terpenoid biosynthesis | Mevalonate kinase | PTS1 |
| K01823 | Terpenoid biosynthesis | Isopentenyl-diphosphate delta-isomerase (type II) (idi1) | |
| K00031 | TCA cycle/glutathione metabolism | Isocitrate dehydrogenase | PTS1 |
| K01438 | Amino acid biosynthesis (arginine) | Acetylornithine deacetylase | PTS1 |
| K01107 | Insositol phosphate metabolism | Inositol polyphosphate 1-phosphatase | |
| K13421 | Pyrimidine metabolism | Orotidine-5-phosphate decarboxylase/Orotate phosphoribosyltransferase | PTS1 |
| K15731 | RNA polymerase II C-terminal domain phosphatase | PTP1-interacting protein, 39 kDa/TFIIF-stimulated CTD phosphatase | PTS1 |
| K09829 | Steroid biosynthesis (ERG2) | C-8 sterol isomerase-like protein | PTS1 |
| K10703 | Long chain fatty acid synthesis | Protein tyrosine phosphatase | |
| N/A | N/A | Thymine-7-hydroxylase | PTS1 |
| N/A | N/A | Hypothetical protein (Q580K0) | PTS1 |
| N/A | N/A | Hypothetical protein (Q389Y7) | |
| N/A | N/A | Hypothetical protein (Q38C56) | |
| N/A | N/A | Hypothetical protein (Q386P8) | PTS1 |
| N/A | N/A | Hypothetical protein(Q388J7) | |
| N/A | N/A | Hypothetical protein (Q38DM9) | |
| N/A | N/A | Hypothetical protein (Q383Q3) | |
| N/A | N/A | Hypothetical protein (Q38AC3) |