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. 2020 Apr 22;9(4):542. doi: 10.3390/plants9040542

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© 2020 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescence in situ hybridization (FISH) mapping of telomeric (TTTAGGG)n sequences and 45S rDNA on mitotic metaphase chromosomes of the tetraploid alfalfa. The extremities of all chromosomes were mapped by the telomeric probe. Note the presence of interstitial telomeric sequences (ITSs) at the centromeric regions of numerous chromosomes; ITSs were also localized in correspondence to nucleolar organizer regions (NORs) (arrows). Insets show the nucleolar chromosomes with signals of the 45S rDNA probe without being overlapped with DAPI. The bar represents 5 µm.