Figure 5.
(A) PCR of DNA extracted from placentas of pregnant buffalo and a bovine bladder tumor. MW: molecular weight marker; lane 1–4: four neoplastic placentas of pregnant buffalo, lane 5: bovine papillomavirus type 2 bladder tumor (positive control), lane 6: buffalo healthy placenta (negative control), lane NTC: no template control. (B) Sequencing results of DNA amplicons. Alignment of the sequences detects a 100% identity with bovine δPV-2 (BPV-2) E5 (Sequence ID: M20219.1).