Table 1.

Gradient profile of mobile phase. Solution A: 0.1% trifluoroacetic acid (TFA) in distilled water, Solution B: 0.1% TFA in acetonitrile.

Elution Time (min)	Solution A (%)	Solution B (%)
4	95	5
12	80	20
14	80	20
22	65	35
25	65	35
30	80	20
35	95	5
55	End of the run