Figure 5.

Subcellular localization of the MEP pathway enzymes in C. roseus chlorophyll-containing cells. C. roseus cells (CR6 cell line) were transiently transformed with plasmids expressing MEP pathway enzymes fused to the N-terminus of GFP as indicated in the first column. The fluorescence emitted by GFP-fused proteins (A1–H1) was compared to chlorophyll autofluorescence (A2–H2). Colocalization of the two signals appeared in yellow while merging the two individual (green/red) color images (A3–H3). Cell morphology was observed with differential interference contrast (DIC, A4–H4). Bar: 10 µm.