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. 2020 Apr 7;9(4):466. doi: 10.3390/plants9040466

Figure 1.

Figure 1

Visualization of intergenic regions used for diagnostic assays of oomycetes with particular emphasis on Phytophthora species. (A) Internal transcribed spacer region, (B) cox1-cox2 spacer region, (C) ras-related ypt1 gene with intronic regions, (D) trnM-trnP-trnM gene order and (E) atp9–nad9 spacer region. Also denoted are the primers used in various reported isothermal (yellow arrows) and non-isothermal (black arrows) assays for Phytophthora species. 1 Phytophthora ramorum-specific primers from Garbelotto et al., 2002. Phyto2 and Phyto3 are nested primers to be used after a preliminary amplification with Phyto1 and Phyto4. 2 Phytophthora ramorum-specific primers from Tomlinson et al., 2010. 3 Phytophthora genus-specific primers from Martin et al., 2004. 4 Phytophthora kernoviae-specific primers from Schena et al., 2006. Yptc3F and Yptc4R are nested primers to be used after a preliminary amplification with YPH1F and YPH2R. 5 Phytophthora infestans-specific primers from Khan et al., 2017. 6 Universal primers for Phytophthora species from Miles et al., 2015. Isothermal assay primers TrnM-F and TrnM-R are used for genus-specific detection. 7 Phytophthora genus-specific (PhyG_ATP9_2FTail and PhyG-R6_Tail, Atp9-F) and species-specific (Psojae-nad9-R for Phytophthora sojae and Psan-nad9-R for Phytophthora sansomeana) primers from Rojas et al., 2017.