Table 4.
Horticulture (Rhododendron sp.) and agriculture (soybean) samples tested with the preformulated lyophilized recombinase polymerase amplification (RPA) assay.
| Phytophthora Species a | Host Plant | Platform | Phytophthora Genus (FAM) | Plant Internal Control (ROX) |
|---|---|---|---|---|
| Mean OT b | Mean OT | |||
| P. boehmeriae | Rhododendron sp. | ViiA7 RT-PCR | 6.78 * | NR |
| P. pseudosyringae | Rhododendron sp. | ViiA7 RT-PCR | 10.63 | 5.92 * |
| P. cryptogea | Rhododendron sp. | ViiA7 RT-PCR | 9.52 | 6.96 |
| P. ramorum | Rhododendron sp. | ViiA7 RT-PCR | 9.7 | 7.41 |
| P. infestans | Rhododendron sp. | ViiA7 RT-PCR | 6.20 | 10.74 * |
| P. infestans | Rhododendron sp. | ViiA7 RT-PCR | 8.01 | 9.84 * |
| Leaf only | Rhododendron sp. | ViiA7 RT-PCR | NR c | 8.15 * |
| Water only | Rhododendron sp. | ViiA7 RT-PCR | NR * | NR * |
| P. sojae 1 | Glycine max | Bio-Rad CFX96 | 8.82 | 11.76 |
| P. sojae 2 | Glycine max | Bio-Rad CFX96 | 6.88 | 8.04 |
| P. sojae 3 | Glycine max | Bio-Rad CFX96 | 5.36 | 7.46 |
| P. sojae 4 | Glycine max | Bio-Rad CFX96 | 4.35 | 7.92 |
| P. sojae 5 | Glycine max | Bio-Rad CFX96 | 3.97 | 10.8 |
| P. sojae 6 | Glycine max | Bio-Rad CFX96 | 10.58 | 13.45 |
| P. sojae 7 | Glycine max | Bio-Rad CFX96 | 9.54 | 15.85 |
| P. sojae 8 | Glycine max | Bio-Rad CFX96 | 9.25 | 12.99 |
| P. sojae 9 | Glycine max | Bio-Rad CFX96 | 4.36 | 27.18 |
| P. sojae 10 | Glycine max | Bio-Rad CFX96 | 5.25 | 22.84 |
a Species of Phytophthora inoculated (ViiA7) or isolated (CFX96) from host. b Values are expressed as the average (n = 2) onset of amplification (OT) in minutes. c (NR) indicates a negative reaction, no different than the water control. * n = 1.