Table 6.

Primers and probes used in this study for Phytophthora genus-specific detection and plant internal control.

Primers, Probes a	Sequence (5′–3′)	Target
Primers
Phytophthora genus specific
TrnM-F	ATGTAGTTTAATGGTAGAGCGTGGGAATC	tRNA-M
TrnM-R	GAACCTACATCTTCAGATTATGAGCCTGATAAG	tRNA-M
Plant internal control
Cox1-IPC-F	CATGCGTGGACCTGGAATGACTATGCATAGA	COX1
Cox1-IPC-R	GGTTGTATTAAAGTTTCGATCGGTTAATAACA	COX1
Probes
Phytophthora genus specific
TrnM-P	TAGAGCGTGGGAATCATAATCCTAATGTTG [FAM-dT] A [THF] G [BHQ1-dT] TCAAATCCTACCATCAT [3′-C3SPACER]	tRNA-M
Plant internal control
Cox1-IPC-P	GGTCCGTTCTAGTGACAGCATTCCYACTTTTATTA [ROX- dT] C [THF] C [BHQ2-dT] YCCGGTACTGGC [3′-C3SPACER]	COX1

^a Primers and probes from Miles et al. 2015 [20].