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. 2020 Apr 23;9(4):311. doi: 10.3390/pathogens9040311

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Decreasing PrP signal after incubation with soils and soil minerals for 7 weeks. Proteins were extracted after 1 (Panel A), 2 (Panel B), 4 (Panel C) or 7 (Panel D) weeks incubation with water (control), quartz, illite, Mte, Luvisolic horizons LFH or Ae, Brunisolic horizons LFH and Bf and Ah horizons from 2 Chernozems. (Panel E): uninfected brain homogenate (BH) incubated for 0, 3 and 10 days. Identical amounts of 1% chronic wasting disease (CWD)-infected or uninfected BH (tgElk-CWD, final concentration 0.25%) were incubated with water (control) soils or soil minerals at 4 °C in silanized tubes. Samples were taken at specified time points and analyzed by western blot using Bar224 antibody.