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. 2020 Apr 23;9(4):311. doi: 10.3390/pathogens9040311

Figure 2.

Figure 2

PrP extracted after incubation with soils or soil minerals. (A) PrP after 1, 5 or 15 weeks incubation with water (control), quartz or illite; (B) PrP after 1, 5 or 15 weeks incubation with Luvisolic horizons LFH or Ae; (C) PrP after 20 weeks incubation with quartz, illite, Mte, Luvisolic horizons LFH or Ae, Brunisolic horizons LFH and Bf and Ah horizons from 2 Chernozems; (D) PrP signal extracted after 30 weeks incubation; (E,F) PrP after incubation with soils and soil minerals for 28 and 100 weeks incubation with water (control), quartz, illite, Mte, Luvisolic horizons LFH and Ae, and Ae horizons from two Podzols. Identical amounts of 1% CWD-infected brain homogenate from tg33 mice ((Panels A–D); final concentration 0.25%) and tgElk mice ((Panels E,F); final concentration 0.25%) were incubated with water (control) or 15 mg/mL suspension of soils/soil minerals at 4 °C in silanized tubes. Samples were taken at specific time points and analyzed by western blot using Bar224 antibody.