Table 2.
Selected reaction monitoring (SRM) condition for the major metabolites of the six uridine 5′-diphosphoglucuronosyl transferase (UGT) enzyme substrates and internal standard (IS).
| UGT Enzyme |
Substrates | Concentration (μM) |
Metabolites | SRM Transition (m/z) |
Polarity | Collision Energy (eV) |
|---|---|---|---|---|---|---|
| 1A1 | SN-38 | 0.5 | SN-38 glucuronide | 569 > 393 | ESI+ | 30 |
| 1A3 | Chenodeoxycholic acid (CDCA) | 2 | CDCA-24 glucuronide | 567 > 391 | ESI− | 20 |
| 1A4 | Trifluoperazine (TFP) | 0.5 | TFP N-glucuronide | 584 > 408 | ESI+ | 30 |
| 1A6 | N-Acetylserotonin (N-SER) | 1 | N-SER glucuronide | 395 > 219 | ESI+ | 10 |
| 1A9 | Mycophenolic acid (MPA) | 0.2 | MPA 7-O-glucuronide | 495 > 319 | ESI− | 25 |
| 2B7 | Naloxone (NX) | 0.2 | NX 3-glucuronide | 504 > 310 | ESI+ | 30 |
| IS | Estrone glucuronide | 0.25 | 445 > 269 | ESI− | 35 |