Table 1.

Comparison of the fifteen Trifolium species.

Species	Genome Length(bp)	GC Content (%)		Gene Density	tRNA	rRNA	mRNA	Genes	Genes with Introns	GenBank Number
	Repetitive %	cp Genome	CDS
T. alexandrinum	148545/2.85%	34.09	37.05	7.54 × 10−4	31	6	75	112	13	MN857160
T. resupinatum	149026/2.69%	33.80	36.64	7.31 × 10−4	37	6	66	109	5	MN857161
T. subterraneum	144763/20.71%	34.83	37.10	7.60 × 10−4	30	4	76	110	16	NC011828
T. meduseum	142595/12.83%	34.87	37.34	7.78 × 10−4	30	4	77	111	15	NC476730.1
T. pratense	121178/NA *	34.63	36.94	7.43 × 10−4	28	4	58	90	11	KJ788290
T. repens	132120/20.70%	34.53	36.96	8.10 × 10−4	31	4	72	107	16	KC894706.1
T.strictum	125834/0.71%	34.98	36.70	8.82 × 10−4	31	5	75	111	18	NC025745.1
T.aureum	126970/5.60%	34.86	36.81	8.51 × 10−4	30	4	74	108	15	KC894708.1
T.boissieri	125740/1.05%	35.24	36.83	8.75 × 10−4	31	5	74	110	17	NC025743.1
T.glanduliferum	126149/0.78%	34.90	36.70	8.72 × 10−4	30	5	75	110	17	NC025744.1
T. grandiflorum	126149/5.60%	37.20	35.82	8.80 × 10−4	30	4	77	111	16	NC_024034
T. hybridum	134831/7.86%	34.33	35.97	8.08 × 10−4	31	4	74	109	17	KJ788286
T. lupinaster	135049/5.98%	33.97	35.71	8.15 × 10−4	30	4	76	110	15	KJ788287
T. occidentale	133780/4.64%	36.34	35.91	8.00 × 10−4	29	4	74	107	17	KJ788289
T. semipilosum	138194/10.55%	36.31	35.81	7.89 × 10−4	31	4	74	109	17	KJ788291

* The cp genome annotation of Trifolium pratense is incomplete, so the percentage of repetitive cannot be calculated [13].