Table 3.
The most abundant tentatively identified saponins in the five Medicago spp. and their quantitative evaluation (% of total saponins).
| Rt | [M-H]− | MS fragments | Tentative identification | % | |
|---|---|---|---|---|---|
| M. heyniana | 17.32 | 825 | 501[M-H-162-162]−(Med) | Med-Hex-Hex | 2.6 |
| 17.80 | 795 | 663[M-H-162]−; 471[M-H-162-162]−(Hed) | Hed-Hex-Hex | 1.6 | |
| 19.14 | 765 | 603[M-H-162]−; 471[M-H-162-132]−(Hed) | Hed-Pen-Hex | 20.3 | |
| 19.26 | 765 | 633[M-H-132]−; 471[M-H-132-162]−(Hed) | Hed-Pen-Hex | 6.8 | |
| 20.08 | 811 | 767[M-H-CO2-H2O]−; 487[M-H-162-162]−(Bayo) | Bayo-Hex-Hex | 16.4 | |
| 21.39 | 811 | 649[M-H-162]−; 487[M-H-162-162]−(Bayo) | Bayo-Hex-Hex | 5.5 | |
| 22.06 | 941 | 879[M-H-CO2-H2O]−;795[M-H-146]−;633[M-H-146-162]−; 457[M-H-146-132-176]−(SoyaB) | Soya B-HexA-Hex-dHex (SSI) | 5.2 | |
| 23.62 | 765 | 603[M-H-162]−; 471[M-H-132-162]−(Hed) | Hed-Pen-Hex | 7.5 | |
| M. hybrida | 15.14 | 1397 | 1265[M-H-132]−; 1011[M-H-CO2-H2O-162-162]−; 455[M-H-CO2-H2O-162-162-146-146-132-132]−(Zanh-CO2-H2O) | Zanh-Hex-Hex-dHex-dHex-Pen-Pen | 24.1 |
| 15.98 | 1235 | 1103[M-H-132]−; 1011[M-H-CO2-H2O-162]−; 455[M-H-CO2-H2O-162-146-146-132-132]−(Zanh-CO2-H2O) | Zanh-Hex-dHex-dHex-Pen-Pen | 3.6 | |
| 16.90 | 825 | 663[M-H-162]−; 601[M-H-162-CO2-H2O]−; 487[M-H-162-176]−(Bayo) | Bayo-HexA-Hex | 7.0 | |
| 17.19 | 971 | 809[M-H-162]−; 633[M-H-162-176]−; 471[M-H-162-176-162]−(Hed) | Hed-HexA-Hex-Hex | 2.0 | |
| 17.51 | 825 | 663[M-H-162]−; 501[M-H-162-162]−(Med) | Med-Hex-Hex | 26.5 | |
| 17.83 | 795 | 663[M-H-162]−; 471[M-H-162-162]−(Hed) | Hed-Hex-Hex | 3.6 | |
| 21.39 | 811 | 649[M-H-162]−; 487[M-H-162-162]−(Bayo) | Bayo-Hex-Hex | 4.3 | |
| 22.05 | 941 | 879[M-H-CO2-H2O]−;795[M-H-146]−; 633[M-H-146-162]−; 457[M-H-146-132-176]−(SoyaB) | Soya B-HexA-Hex-dHex (SSI) | 1.2 | |
| 22.56 | 795 | 633[M-H-162]−; 471[M-H-162-162]−(Hed) | Hed-Hex-Hex | 4.0 | |
| M. lupulina | 15.90 | 1103 | 927[M-H-176]−; 909[M-H-176-H2O]−; 517[M-H-176-410]−(Zanh) | Zanh-HexA-dHex-Pen-Pen | 6.1 |
| 17.63 | 1219 | 1043[M-H-176]−; 911[M-H-176-132]−; 501[M-H-176-132-410]−(Med) | Med-HexA-dHex-Pen-Pen-Pen | 9.2 | |
| 17.87 | 1087 | 911[M-H-176]−; 501[M-H-176-410]−(Medic) | Med-HexA-dHex-Pen-Pen | 45.8 | |
| 21.96 | 911 | 765[M-H-146]−; 457[M-H-146-132-176]−(SoyaB) | Soya B-HexA-Pen-dHex | 13.0 | |
| 22.05 | 941 | 879[M-H-CO2-H2O]−;795[M-H-146]−;633[M-H-146-162]−; 457[M-H-146-132-176]−(SoyaB) | Soya B-HexA-Hex-dHex (SSI) | 7.2 | |
| M. murex | 17.10 | 971 | 809[M-H-162]−; 663[M-H-162-146]−; 487[M-H-162-146-176]−(Bayo) | Bayo-HexA-dHex-Hex | 3.2 |
| 18.92 | 955 | 793[M-H-162]−; 647[M-H-162-146]−; 471[M-H-162-146-176]−(Hed) | Hed-HexA-Pen-Hex | 7.7 | |
| 20.09 | 811 | 767[M-H-CO2-H2O]−; 487[M-H-162-162]−(Bayo) | Bayo-Hex-Hex | 44.1 | |
| 22.03 | 941 | 879[M-H-CO2-H2O]−;795[M-H-146]−;633[M-H-146-162]−; 457[M-H-146-132-176]−(SoyaB) | Soya B-HexA-Hex-dHex (SSI) | 28.7 | |
| 22.56 | 795 | 633[M-H-162]−; 471[M-H-162-162]−(Hed) | Hed-Hex-Hex | 1.5 | |
| 22.85 | 765 | 619[M-H-146]−; 487[M-H-146-132]−(Bayo) | Bayo-dHex-Pen | 5.3 | |
| 23.64 | 765 | 603[M-H-162]−; 471[M-H-132-162]−(Hed) | Hed-Hex-Pen | 4.0 | |
| M. truncatula | 15.70 | 1383 | 997[M-H-162-162-CO2-H2O]−; 841[M-H-132-410]−; 455[M-H-CO2-H2O-132-410-162-162]−(Zanh-CO2-H2O) | Zanh-Hex-Hex-Pen-dHex-Pen-Pen | 3.1 |
| 15.82 | 1383 | 997[M-H-162-162-CO2-H2O]−; 841[M-H-132-410]−; 455[M-H-CO2-H2O-132-410-162-162]−(Zanh-CO2-H2O) | Zanh-Hex-Hex-Pen-dHex-Pen-Pen | 11.9 | |
| 17.56 | 1367 | 1235[M-H-132]−; 981[M-H-162-162-CO2-H2O]−; 849[M-H-162-162-CO2-H2O-132]−; 439(Med-CO2-H2O) | Med-Hex-Hex-Pen-dHex-Pen-Pen | 8.9 | |
| 17.87 | 1087 | 911[M-H-176]−; 677[M-H-410]−; 501[M-H-176-410]−(Med) | Med-HexA-Pen-dHex-Pen | 2.3 | |
| 21.96 | 911 | 765[M-H-146]−; 457[M-H-146-132-176]−(SoyaB) | Soya B-HexA-Pen-dHex | 9.0 | |
| 22.05 | 941 | 879[M-H-CO2-H2O]−;795[M-H-146]−;633[M-H-146-162]−; 457[M-H-146-132-176]−(SoyaB) | Soya B-HexA-Hex-dHex (SSI) | 54.2 |