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In vitro biological activities of GLP1_C, GLP1_16HSA, GLP1_19HSA, and GLP1_28HSA determined in GLP-1R-expressing cells. GLP-1R-expressing HEK cells were treated with increasing concentrations of GLP1_C, GLP1_16HSA, GLP1_19HSA, or GLP1_28HSA. The y-axis values were converted to percentages by averaging the cAMP production of 10−7 M GLP1_C. Each data point represents the mean (n = 3) ± SD.
