Figure 2.

Effect of BJ on cytotoxicity induced by stressors. Cell viability was detected with the 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) assay (A,B). The change of viability was determined as percentage of viable cells in treated cultures compared to those in untreated ones. Cell death was evaluated by the trypan blue (TB) assay and expressed as percentage of non-viable (blue stained) vs. total cells counted (C,D). Results are showed as means ± S.E.M. from three independent experiments performed eight-fold (MTT) or in triplicate (TB). * P < 0.05, ** P< 0.01 and *** P < 0.001 vs. ctrl; °° P < 0.01 and °°° P < 0.001 vs. 6-hydroxydopamine (6-OHDA) or H₂O₂ 50 µM; ^§ P < 0.05, ^§§ P < 0.01 and ^§§§ P < 0.001 vs. 6-OHDA 50 µM or H₂O₂ + BJ 0.5%; ^ P < 0.05, ^^ P < 0.01 and ^^^ P < 0.001 vs. 6-OHDA or H₂O₂ 100 µM; ⁺ P < 0.05, ⁺⁺ P < 0.01 and ⁺⁺⁺ P < 0.001 vs. 6-OHDA 100 µM or H₂O₂ + BJ 0.5%; ^££ P < 0.01 vs. H₂O₂ 150 µM; ^## P < 0.01 vs. H₂O₂ 150 µM + BJ 0.5%.