Figure 2.

Hepatitis E virus (HEV) RNA kinetic in PHESCs challenged with HEV preparations. PHESCs, isolated from different donors, were challenged with stool-derived HEV-1 (upper panels) or stool-derived HEV-3 (lower panels), then the lysis of cells and/or collection of supernatants were done at 0, 1, 3, 5, 7, 10, 12, 14 and 21 days post-infection. (A) represents PHESCs isolated from donor #1, (B) represents PHESCs isolated from donor #2, and (C) represents PHESCs isolated from donor #3. The HEV RNA load was quantified in these samples by qPCR. The solid line represents intracellular HEV RNA load, the dotted line represents extracellular RNA load, and LOQ is the limit of quantification. Depicted are the mean values of three independent experiments ± SEM.