Figure 4.

HEV-1 upregulated the inflammatory transcriptome and impaired the expression of Impaired Interferon (IFN) type III in PHESCs. PHESCs were challenged with HEV-1 and/or HEV-3 for 7 and 10 days, respectively, and then total RNA was extracted and the expression level of mRNA of proinflammatory markers (IL-6, Cxcl-9, and Cxcl-11) (A–C) and IFN transcripts (IFN-λ1 and IFN-λ2/3) (D,E) was normalized to the housekeeping gene (18srRNA). The relative gene expression was determined by comparing the expression levels of these transcripts with mock cells. The data represent the mean +/- SEM of four separate experiments. * indicates p ≤ 0.05, as assayed by a two-tailed Student’s t-test