Table 2.
Target genes and sequences of primers used in qPCR analysis.
| Gene | Primer Sequence 5′-3′ | Product Size (bp) |
|---|---|---|
| 18srRNA | Forward GTAACCCGTTGAACCCCATT Reverse CCATCCAATCGGTAGTAGCG |
151 |
| IL-6 | Forward TCAATATTAGAGTCTCAACCCCCA Reverse TTCTCTTTCGTTCCCGGTGG |
90 |
| IL-8 | Forward ATGACTTCCAAGCTGGCCGTGGCT Reverse TCTCAGCCCTCTTCAAAAACTTCTC |
292 |
| IL-15 | Forward: CTGACGTCACATGGAGCACA Reverse: CTGCACTGAAACAGCCCAAA |
283 |
| IL- β1 |
Forward CCACAGACCTTCCAGGAGAATG Reverse GTGCAGTTCAGTGATCGTACAGG |
131 |
| MCP-1 (CCL2) |
Forward AGTCTCTGCCGCCCTTCT Reverse GTGACTGGGGCATTGATTG |
93 |
| TNF-α |
Forward CGCTCCCCAAGAAGACAG Reverse AGAGGCTGAGGAACAAGCAC |
60 |
| Cxcl-9 |
Forward AGTGCAAGGAACCCCAGTAG Reverse AGGGCTTGGGGCAAATTGTT |
112 |
| Cxcl-10 | Forward: CCACGTGTTGAGATCATTGCT Reverse: TGCATCGATTTTGCTCCCCT |
152 |
| Cxcl-11 |
Forward:
GAGTGTGAAGGGCATGGCTA
Reverse: ACATGGGGAAGCCTTGAACA |
71 |
| IFN-α |
Forward:
CCTGATGAATGCGGACTCCA
Reverse: TAGCAGGGGTGAGAGTCTTTG |
265 |
| IFN-β |
Forward: CGCCGCATTGACCATCTA.
Reverse: GACATTAGCCAGGAGGTTCTC. |
112 |
| IFN-ɤ | Forward GAGTGTGGAGACCATCAAGGAAG Reverse TGCTTTGCGTTGGACATTCAAGTC |
124 |
| IFN-λ1 | Forward: GCAGGTTCAAATCTCTGTCACC Reverse AAGACAGGAGAGCTGCAACTC |
109 |
| IFN-λ2/3 | Forward: CAGCTGCAGGTGAGGGA Reverse GCGGTGGCCTCCAGAACCTT |
77 |