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. 2020 Apr 24;9(4):316. doi: 10.3390/pathogens9040316

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical VSV-staining of orally infected C. sonorensis females. (A) Ovaries (Ov) and (D) abdomen of non-infected (negative control) females. (B) Ovaries (Ov) and (C,E,F) abdominal sections from females 9–13 days post feeding with VSV-positive antigen staining of viral nucleocapsid in red and counterstained with hematoxylin (blue). (B) VSV antigen staining in the ovarial sheaths and trachea. (C) VSV antigen staining in hindgut (Hg), gonopore (Gp), gonotreme (Go), lateral oviduct (LOd), spermathecal duct (Sd), and ovaries (Ov). (E) VSV antigen staining in hindgut (Hg), gonopore (Gp), gonotreme (Go), and lateral oviduct (LOd), with negative ovaries (Ov) and spermatheca (S). (F) VSV antigen staining in rectum (R) and gonotreme (Go), with negative spermatheca (S).