Table 1.
Venereal transmission rates from orally infected females to naïve non-infected males during three gonotrophic cycles (GC) as detected by whole-body RT-qPCR or CPE.
| GC | Initial Number of Midges | Surviving ♂ (%) 1 | VSV RT-qPCR+ ♂ (%) 2 | VSV CPE+ ♂ (%) 3 |
|---|---|---|---|---|
| 1 | 240 ♀ and 120 ♂ | 88/120 (73.3%) | 0 | ND |
| 2 | 199 ♀ and 100 ♂ | 59/100 (59%) | 9/59 (15.2%) | ND |
| 2 | 104 ♀ and 52 ♂ | 30/52 (57.7%) | ND | 6/30 (20%) |
| 3 | 131 ♀ and 65 ♂ | 38/65 (58.5%) | 29/38 (76.3%) | ND |
| 3 | 64 ♀ and 32 ♂ | 19/32 (59.4%) | ND | 6/19 (31.6%) |
1 Surviving males were sampled 4 days after initial cohabitation, which was determined as the end of each blood meal-induced gonotrophic cycle (GC). 2 Vesicular stomatitis virus (VSV)-positive males detected by RT-qPCR. 3 VSV-positive males detected by cytopathic effect (CPE). ND, not determined.