Table 3.
Ethidium bromide monoazide quantitative polymerase chain reaction (EMA-qPCR) analysis of photodynamically damaged C. difficile KCTC 5009 DNA.
| DNA Samples | Ct Values (Mean ± SD, n = 3) |
|---|---|
| Control a | 14.67 ± 0.22 |
| UVA b | 15.60 ± 0.00 |
| Chitosan c | 17.46 ± 0.12 |
| UVA + chitosan d | 19.45 ± 0.05 |
| UVA + TC e | 20.46 ± 0.12 |
| UVA + TC + chitosan f | 24.17 ± 0.08 |
| UVA + DXY e | 21.43 ± 0.01 |
| UVA + DXY + chitosan f | 25.28 ± 0.01 |
| UVA + MIN e | 20.25 ± 0.03 |
| UVA + MIN + chitosan f | 25.31 ± 0.03 |
| UVA + TGE e | 21.38 ± 0.10 |
| UVA + TGE + chitosan f | 25.54 ± 0.17 |
a = DNA from C. difficile KCTC5009 without TC, Chitosan, and UVA; b = DNA from C. difficile KCTC5009 treated with UVA only; c = DNA from C. difficile KCTC5009 treated with chitosan (0.0125%) only; d = DNA from C. difficile KCTC5009 treated with UVA and chitosan (0.0125%); e = DNA from C. difficile KCTC5009 treated with UVA and each of the four TCs; f = DNA from C. difficile KCTC5009 treated with UVA, each of the four TCs, and chitosan (0.0125%).